When we analyzed the results of the lab, one of our experimental samples contained a different dye that didn't match the reference dyes. I think that the Fast Green FCF would migrate similarly to the dyes that we examined in this lab, because I think it is negatively charged which was like the samples that we used which were the dyes from candies. I think that dog food manufacturers put artificial food coloring in dog foods because it may be quicker and easier and more pleasant to look at than having something that takes long to make or something that doesn't look appealing. I think that artificial colors are more preferable than natural food colors because maybe the colors make it more appealing than natural food colors and it is easier to use and get.
The two factors that controlled the distance the colored dye solutions migrated were the charges from the gel electrophoresis chamber and how the dyes from the candies charges. The force that helped move the dyes through the gel was electricity. The component of the electrophoresis system that caused the molecules to separate by size was the agarose in the gel, because the dyes were placed in the wells in the gel so it made the molecules of the dyes separate after the electrophoresis chamber was turned on. I expect that DNA molecules with molecular weights of 600, 1000, 2000, and 5000 daltons would separate the same as the dyes that we used but it probably won't move as quickly or travel as far as the dyes that we experimented with during this lab.
No comments:
Post a Comment