Monday, January 30, 2017

pGLO Lab Analysis




Plate
Number of Colonies
Color of colonies under room light
Color of colonies under   UV light
- pGLO LB
0nonenone
- pGLO LB/amp
0nonenone
+ pGLO LB/amp
39whitenone
+ pGLO LB/amp/ara
31whitebright green, glowing


The two new traits that our transformed bacteria had was that under UV light that colonies glowed and it was resistant to ampicillin. I think that in the 100 uL of bacteria that we spread on the plate there was about 30 million bacteria, because you usually have to look through microscopes in order to see one bacteria. So probably many bacteria could fit in that single colony. The role of arabinose in the plates was that the bacteria would only glow if it was present.

In the website addgene.org, three current uses for GFP(green fluorescent protein) are fusion tagging, protein purification, and biosensors. Other applications for genetic engineering are producing insulin and human growth hormones.
































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